gga-miR-155对MDCC-MSB1细胞生物学行为的影响

doi:10.11843/j.issn.0366-6964.2018.11.022

Abstract

Abstract:

In order to detect the effect of gga-miR-155 on the biological behavior of MDCC-MSB1 cells, MDCC-MSB1 cells were transiently transfected with gga-miR-155 mimic, gga-miR-155 inhibitor, and negative control which were artificial synthesized. Then, the expression of gga-miR-155 was detected by real-time PCR (qRT-PCR); the cell proliferation was detected by CCK-8 assay; the cell cycle and apoptosis were analyzed by flow cytometry; and the cell migration and invasive ability were determined by transwell assay. Results showed that gga-miR-155 mimic up-regulated the expression level of gga-miR-155 in MDCC-MSB1 cells. After gga-miR-155 mimic transfection, the proliferation of MDCC-MSB1 cells was promoted, G1 phase cells were decreased, S and G2 cells were increased, the apoptosis rate was reduced,the migration invasion capability were increased in gga-miR-155 mimic transfected cells. On the contrary, the gga-miR-155 inhibitor significantly down-regulated the expression level of gga-miR-155 in MDCC-MSB1 cells. When the gga-miR-155 inhibitor was transfected into MDCC-MSB1 cells, cell proliferation was inhibited,G1 phase cells were increased, S and G2 cells were reduced, the cell apoptosis was increased, and the migration invasion capability were suppressed. The results showed that gga-miR-155 could promote the proliferation, migration and invasion of MDCC-MSB1 cells and inhibit their apoptosis.

CLC Number:

YU Zu-hua, DING Ke, YU Chuan, JIA Yan-yan, HE Lei, LIAO Cheng-shui, LI Jing, ZHANG Chun-jie, LI Yin-ju, WU Ting-cai, CHENG Xiang-chao, ZHANG Meng-ke, QIU Jing-jing, LUO Jun. Effect of gga-miR-155 on the Biological Behavior of MDCC-MSB1 Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2496-2504.

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Effect of gga-miR-155 on the Biological Behavior of MDCC-MSB1 Cells

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